Difference in DNA Metabolism between Tumor and Normal Tissues

概要

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Two fractions having thymidine kinase (ATP; thymidine 5 phosphotransferase, EC 2. 7. 1. 21) activity were separated from tumor cells such as Yoshida sarcoma, Ehrlich ascites tumor, Hepatoma AH 130, Morris hepatoma 7793 and Morris hepatoma 7794 A by means of DEAE-cellulose column chromatography, while only a single fraction of thymidine kinase was observed in normal cells such as bone marrow, spleen, embryonic and regenerating rat liver.<BR>The properties of thymidine kinases from Yoshida sarcoma were compared with those of the regenerating liver thymidine kinase.<BR>By the properties we meant Km for thymidine and for ATP, heat stability, urea effect and optimal pH.<BR>DEAE-Cellulose column chromatography of purified thymidine kinase from the Yoshida sarcoma yields two peaks of enzyme activity.<BR>Molecular weight of Peak I and Peak II have been estimated to be 70,000 and 120,000, respectively.
Japan Society of Clinical Chemistryの論文