Pseudocholinesterase with Different Type of Inhibition:Ch-E Measurement Using New Substrate (p-Hydroxybenzoylcholine)

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Human serum cholinesterase activity and its inhibition ratios with dibucaine and fluoride were measured by butyrylthiocholine-5, 5'OE-dithio-bis-2-nitrobenzoic acid (BTC-DTNB) method for 1096 samples. As the result of these samples measurement, only one sample showed approximately 10% lower inhibition ratio than others. This sample showed also 10% lower inhibition ratio by both the Kalow's benzoylcholine UV method and p-hydroxybenzoylcholine (p-HBC) UV method. According to the p-HBC UV method, the mean value of cholinesterase activity was 266.4 IU/1 (SD=81.1) out of 1096 samples. Six human serums from the same family including the above exceptional case were examined for dibucaine and fluoride inhibition ratio by p-HBC UV method. The result showed that all samples excepting the above mentioned case were normal level in the inhibition ratio. Furthermore, the polyacrylamide electrophorersis of this sample revealed the disappearance of both C1 and C2 bands in electrophoretic pattern.
Japan Society of Clinical Chemistryの論文