A New Simple and Rapid Method for Determination of Total and Direct Serum Bilirubin by Use of 1, 5-Naphthalene Disulfonate of Diazo Sulfamilic Acid

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A rapid and simple method for determination of both total and direct bilirubin is reported.The reagent consists of 1, 5-naphthalene disulfonate of diazosulfanilic acid and dimethylsulfoxide as an accelerator of the reaction.The reagent develops an endpoint of reaction within 15 and 10min at room temperature and 37°C, respectively.The developed color of reaction mixture, which was found to be stable at least for 1h, has its maximum absorption at560nm.The influence of hemoglobin on the bilirubin determination was demonstrated to be smaller for the present method than other two conventional methods employing diazotization reagents.The results were well correlated with those obtained by both manual (Jendrassik-Grof and Malloy-Evelyn) and automated (SMA 12/60) methods. In addition, this new method can be applied to automated analysis with either discrete type or centrifugal analyzers.
Japan Society of Clinical Chemistryの論文