A Simple Enzymatic Method for Determination of Lipid Compo8itions of Human Serum Lipoproteins separated by Agarose Gel Electrophoresis

概要

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A simple enzymatic method is described for determination of lipid compositions of human serum lipoprotein fractions separated by agarose gel eiectrophoresis. Human serum lipoproteins were separated by electrophoresis using a agarose gel with four sample troghs or four gelplates.<BR>Thereafter, one of the plates was stained with Sudan Black B for WHO Typing of hyperlipoprotehemia, and the others were stained separately with commercially available enzymatic reagents for determination of serum triglyceride, total cholesterol and phospholipids, during a short time to recognize the position of lipoprotein fractions in the gels. And then, each lipoprotein fraction area was cut out of the gels and stainLd further in tubes which contained the enzymatic reagents. After staining, the distribution ratios of these three lipids to each lipoprotein fraction were separately calculated from the ratios of each fraction to the total fractions in the optical density with regards to each lipid. Then, the lipid compositions of each fraction were determined by multiplying of these distribution ratios with each lipid concentration in serum. This method is simple to perform without any special instrument, and requires only small amount of sample. it also has sufficient reproducibility, specificity and sensitivit.
Japan Society of Clinical Chemistryの論文