Comparative Studies on Angiotensins

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概要

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• We have examined angiotensin-like substances produced by incubating renal and extrarenal tissue extracts with homologous plasmas in representative species of vertebrate classes comprising from teleosts to mammals. SE-Sephadex column chromatograms, ratios of oxytocic to pressor activity (O: P ratio), and susceptibilities to proteases were used as the criteria. Angiotensin-like substances from renal and extrarenal tissues in mammals did not differ from Asp<SUP>1</SUP>-Ile<SUP>5</SUP>-angiotensin I. The active substance derived from avian kidney showed a peak at a more acid site in the chromatogram, a low O: P ratio, and greater susceptibility to proteases. The substance from reptilian kidney was eluted at a position similar to Asp<SUP>1</SUP>-Ile<SUP>5</SUP>-angiotensin I, but it had an increased O: P ratio. It was also more resistant to enzyme treatments. The substance from amphibian kidneys was also eluted at a similar position, but a minor activity appeared later. The main peak for bullfrog material had a lower oxytocic activity, and was more susceptible to proteases. In teleosts, two peaks were always seen in the chromatograms. They were more susceptible to proteases than known angiotensins I or II. Some peaks had increased the oxytocic activity. The substances produced by aglomerular kidney of <I>Lophius litulon</I> and by the corpuscles of Stannius of carp had a common peak at an extremely basic site. Angiotensin-like substances of avian, reptilian, amphibian and teleostean origins are probably different from the known angiotensins I or II.<BR>Among these unknown angiotensins, rat angiotensin was employed as a test material for further idenfication by dansyl procedure. The dansyl derivative or rat angiotensin and its tryptic or chymotryptic fragments showed the same chromatographical behaviors as that of Asp<SUP>1</SUP>-Ile<SUP>5</SUP>-angiotensin I on a thin layer of Silica gel H. This result shows that angiotensin of human, hog, horse and rat, except bovine, has the same sequence. The dansyl method was capable enough with 5 pg of the peptide.

• Japan Society of Clinical Chemistryの論文

著者

• 中嶋 暉躬

  広島大学医学部総合薬学科

• 曽我部 博文

  東邦大学医学部薬理学

• 中山 妙子

  広島大学医学部薬学科

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