Measurement of Cyclic Nucleotide Contents and Cyclase Activities in the Regenerating Rat Liver by Enzyme Immunoassay

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After partial hepatectomy (about 70%) in rats, the activities of adenylate cyclase, guanylate cyclase, and cytidylate cyclase and contents of cAMP, cGMP, and cCMP in the regenerating livers were assayed by enzyme immunoassays, which were recently established by our laboratory.<BR>cAMP contents in regenerating livers at 4-16 hr after hepatectomy were about 2.5-3 times greater than those in normal livers, but adenylate cyclase activities were not changed during this experimental period. cCMP contents and guanylate cyclase activities were almost the same levels as prehepatectomy levels. cCMP contents in the regenerating liver has been shown to increase at 4 hr and 16 hr after hepatectomy in biphasic manner. Cytidylate cyclase activities remain unchanged.<BR>These findings were identical to those reported by other investigators using RIA technique. It was confirmed that cyclic nucleotides were exactly and simply assayed by these enzyme immunoassays.
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