Suppressive Effects of Oral Administration of Heat-killed Lactobacillus acidophilus on Th17 Immune Responses in a Bovine β-lactoglobulin- sensitized Mice Model

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概要

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• The present study was planned to explore the effect of L.acidophilus on the Th17 immune response in a mouse model of β-lactoglobulin (β-lg) allergy.Bovine β-lg sensitised BALB/c mice were orally administered with different doses of heat-killed L. acidophilus (low, 5×107 CFU; medium, 5×108CFU; high, 5×109CFU) in 200 μL of PBS three times a week, starting from 1 weeks before β-lg sensitisation for 4 weeks. After the allergen challenge, the numbers of blood eosinophils and neutrophils were examined by light microscope; the levels of cytokine (IL-12, IL-4, TGF-β, IL-10, IL-6 and IL-17A), total IgE and β-lg-specific IgE contents in the serum were measured with ELISA; The mRNA expression levels of TGF-β, IL-17A,CD25, Foxp3, RORγt and IL-10 were analyzed using real-time PCR.The results showed that oral administration of L. acidophilus suppressed hypersensitivity responses, attenuated the numbers of inflammatory cells and inhibited IgE production. We found up-regulation of TGF-β and down-regulation of IL-17A in the serum of L. acidophilus-treated group, along with IL-6 levels was significantly decreased than that of the allergy group (P<0.05). Moreover, the mRNA expression levels of CD25, Foxp3 and TGF-β were significantly higher in the spleen of L.acidophilus-treated group, while the mRNA expression levels of IL-17A, RORγt and IL-10 were significantly lower than that in the allergy group (P<0.05).In conclusion, the suppression of major allergic symptoms by oral administration of L. acidophilus was probably due to improve the Treg/Th17 balance and inhibit the IL-6 production.

著者

• Li Dan

  Key Laboratory of Agricultural Products Processing in Jilin Provincial Universities, Changchun University

• Meng Xiang-chen

  Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agriculture University

• Li Ai-li

  Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agriculture University

• Duan Cui-cui

  Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agriculture University

• Huo Gui-cheng

  Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agriculture University

• Zheng Quan-ling

  Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agriculture University

関連論文

• Suppressive Effects of Oral Administration of Heat-killed Lactobacillus acidophilus on Th17 Immune Responses in a Bovine β-lactoglobulin- sensitized Mice Model
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