Individual chromosome identification, chromosomal collinearity and genetic-physical integrated map in Gossypium darwinii and four D genome cotton species revealed by BAC-FISH
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概要
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The study was conducted on the individual chromosome identification in Gossypium darwinii (AdDd), G. klotzschianum (D3k), G. davidsonii (D3d), G. armourianum (D2-1) and G. aridum (D4) using a multi-probe fluorescence of in situ hybridization (FISH) system. Comparative analysis on their genetic maps with that of physical maps was made as well. The FISH probes used contained two sets of bacterial artificial chromosome (BAC) clones [one is specific to 26 individual chromosomes from A and D subgenomes of G. hirsutum (Ah and Dh) while the other is a D genome centromere-specific BAC clone 150D24], 45S and 5S rDNA clones. The results showed that all Ad chromosomes were marked with the 13 Ah chromosome-specific BAC clones, whilst all Dd, D3k, D3d, D2-1 and D4 chromosomes and chromosomal arms were identified with the 13 Dh chromosome-specific BAC clones and the D genome centromere-specific BAC. According to the homology within D subgenomes which are between A (D) genome and A (D) subgenome, the systematic nomenclature for individual chromosome in the five species was established. The chromosomes of A (D) subgenomes in G. darwinii were named as Ad01-Ad13 (Dd01-Dd13). The chromosomes in D3k, D3d, D2-1 and D4 were named as D3k01-D3k13, D3d01-D3d13, D2-101-D2-113 and D401-D413, respectively. Based on the successful identification for individual chromosomes, 45S and 5S rDNA were located to the special chromosomes and chromosomal arms for all five species. And there appeared chromosomal collinearity from the BAC clones among different species by comparing BACs positions, which suggested that the majority of chromosome segment homology may exist between D genomes and D subgenome. Moreover, as the genetic map and physical map were integrated, the orientations of genetic maps for Dd and D genomes of diploid cotton were established. The orientations of some of chromosomes in genetic maps (Dd03, Dd04, Dd06, Dd09, Dd10 and Dd12) were found switched. The SSR marker in the middle of linkage group 04 was corrected at nearby the end of chromosome 04 by FISH. The study will be helpful to establish a theoretical basis using the wild gene bank to exploit more genes aiming for cotton breeding and will provide powerful evidences both for the evolution of Gossypium and assembling the sequences of the obtained and as well the on-going whole genome sequencing projects of cotton.
著者
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Wang Yuhong
State Key Laboratory Of Cotton Biology China And Cotton Research Institute Of Chinese Academy Of Agr
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Zhang Xiangdi
State Key Laboratory Of Cotton Biology China And Cotton Research Institute Of Chinese Academy Of Agr
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Wang Kunbo
State Key Laboratory Of Cotton Biology China And Cotton Research Institute Of Chinese Academy Of Agr
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Liu Fang
State Key Lab For Powder Metallurgy Central South University
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Peng Renhai
State Key Laboratory of Cotton Biology (China)/Cotton Research Institute of Chinese Academy of Agricultural Science
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Gan Yimei
State Key Laboratory of Cotton Biology (China)/Cotton Research Institute of Chinese Academy of Agricultural Science
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Wang Chunying
State Key Laboratory of Cotton Biology (China)/Cotton Research Institute of Chinese Academy of Agricultural Science
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Li Shaohui
State Key Laboratory of Cotton Biology (China)/Cotton Research Institute of Chinese Academy of Agricultural Science
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Zhang Xiangdi
State Key Laboratory of Cotton Biology (China)/Cotton Research Institute of Chinese Academy of Agricultural Science
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Wang Kunbo
State Key Laboratory of Cotton Biology (China)/Cotton Research Institute of Chinese Academy of Agricultural Science
関連論文
- Individual chromosome assignment and chromosomal collinearity in Gossypium thurberi, G. trilobum and D subgenome of G. barbadense revealed by BAC-FISH
- Effect of Molybdenum-Rich Carbides on Wear Behavior of Powder Metallurgy Steel
- Individual chromosome identification, chromosomal collinearity and genetic-physical integrated map in Gossypium darwinii and four D genome cotton species revealed by BAC-FISH
- Individual chromosome identification, chromosomal collinearity and genetic-physical integrated map in Gossypium darwinii and four D genome cotton species revealed by BAC-FISH