Individual chromosome assignment and chromosomal collinearity in Gossypium thurberi, G. trilobum and D subgenome of G. barbadense revealed by BAC-FISH
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概要
- 論文の詳細を見る
The experiment on individual chromosome assignments and chromosomal diversity was conducted using a multi-probe fluorescence in situ hybridization (FISH) system in D subgenome of tetraploid Gossypium barbadense (Db), G. thurberi (D1) and G. trilobum (D8), which the later two were the possible subgenome donors of tetraploid cottons. The FISH probes contained a set of bacterial artificial chromosome (BAC) clones specific to 13 individual chromosomes from D subgenome of G. hirsutum (Dh), a D genome centromere-specific BAC clone 150D24, 45S and 5S ribosomal DNA (rDNA) clones, respectively. All tested chromosome orientations were confirmed by the centromere-specific BAC probe. In D1 and D8, four 45S rDNA loci were found assigning at the end of the short arm of chromosomes 03, 07, 09 and 11, while one 5S rDNA locus was successfully marked at pericentromeric region of the short arm of chromosome 09. In Db, three 45S rDNA loci and two 5S rDNA loci were found out. Among them, two 45S rDNA loci were located at the terminal of the short arm of chromosomes Db07 and Db09, whilst one 5S rDNA locus was found situating near centromeric region of the short arm of chromosome Db09. The positions of the BAC clones specific to the 13 individual chromosomes from Dh were compared between D1, D8 and Db. The result showed the existence of chromosomal collinearity within D1 and D8, and as well between them and Db. The results will serve as a base for understanding chromosome structure of cotton and polyploidy evolution of cotton genome and will provide bio-information for assembling the sequences of finished and the on-going cotton whole genome sequencing projects.
- 2011-06-25
著者
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Liu Fang
State Key Laboratory Of Cotton Biology China And Cotton Research Institute Of Chinese Academy Of Agr
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GAN Yimei
College of Plant Science and Technology, Huazhong Agricultural University
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CHEN Dan
State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Ag
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WANG Chunying
State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Ag
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LI Shaohui
State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Ag
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ZHANG Xiangdi
State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Ag
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WANG Yuhong
State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Ag
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PENG Renhai
State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Ag
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WANG Kunbo
State Key Laboratory of Cotton Biology, China and Cotton Research Institute of Chinese Academy of Ag
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Gan Yimei
College Of Plant Science And Technology Huazhong Agricultural University
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Wang Yuhong
State Key Laboratory Of Cotton Biology China And Cotton Research Institute Of Chinese Academy Of Agr
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Peng Renhai
State Key Laboratory Of Cotton Biology China And Cotton Research Institute Of Chinese Academy Of Agr
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Zhang Xiangdi
State Key Laboratory Of Cotton Biology China And Cotton Research Institute Of Chinese Academy Of Agr
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Wang Chunying
State Key Laboratory Of Cotton Biology China And Cotton Research Institute Of Chinese Academy Of Agr
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Chen Dan
State Key Laboratory Of Cotton Biology China And Cotton Research Institute Of Chinese Academy Of Agr
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Wang Kunbo
State Key Laboratory Of Cotton Biology China And Cotton Research Institute Of Chinese Academy Of Agr
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Li Shaohui
State Key Laboratory Of Cotton Biology China And Cotton Research Institute Of Chinese Academy Of Agr
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Liu Fang
State Key Lab For Powder Metallurgy Central South University
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Peng Renhai
State Key Laboratory of Cotton Biology (China)/Cotton Research Institute of Chinese Academy of Agricultural Science
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Wang Chunying
State Key Laboratory of Cotton Biology (China)/Cotton Research Institute of Chinese Academy of Agricultural Science
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Li Shaohui
State Key Laboratory of Cotton Biology (China)/Cotton Research Institute of Chinese Academy of Agricultural Science
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Zhang Xiangdi
State Key Laboratory of Cotton Biology (China)/Cotton Research Institute of Chinese Academy of Agricultural Science
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Wang Yuhong
State Key Laboratory of Cotton Biology (China)/Cotton Research Institute of Chinese Academy of Agricultural Science
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Wang Kunbo
State Key Laboratory of Cotton Biology (China)/Cotton Research Institute of Chinese Academy of Agricultural Science
関連論文
- Individual chromosome assignment and chromosomal collinearity in Gossypium thurberi, G. trilobum and D subgenome of G. barbadense revealed by BAC-FISH
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