フェニルボロン酸単分子膜修飾電極のリボヌクレオシド応答性

概要

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The surface of a gold (Au) disk electrode was modified with a self-assembled monolayer consisting of phenylboronic acid residue to prepare a ribonucleoside-sensitive electrode. The cyclic voltammetric response of the modified electrode to [Fe(CN)6]3− ion dissolved in the sample solution was found to be dependent of the type of ribonucleoside and its concentration. In other words, the peak current in the cyclic voltammogram of [Fe(CN)6]3− ion was significantly decreased when uridine or cytidine was added in the working buffer, while the response to thymidine, 2′-deoxyuridine, and 2′-deoxycytidine was rather small. The voltammetric response was rationalized on the basis of the electrostatic repulsion between [Fe(CN)6]3− ion and the negatively-charged electrode surface, which originates from the addition of OH− ion on the electrode as a result of the formation of phenylboronic acid-ribonucleoside conjugate on the surface of the monolayer.
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