Cell-Based Electrochemical Assay for Endotoxin Using a Secreted Alkaline Phosphatase Reporter System

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概要

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• We developed a cell-based assay device for the detection of endotoxin, the potentially toxic compound that induces septic shock. Genetically-engineered cells that secrete alkaline phosphatase (SEAP) on exposure to endotoxin were cultured in an electrochemical cell device in medium containing p-aminophenyl phosphate and various concentrations of endotoxin. After 24 hr incubation, p-aminophenol (pAP), generated by SEAP-catalyzed hydrolysis, was detected by amperometry at +0.35 V. The amperometric response increased with the concentration of endotoxin in the range of 0.01–1 ng/ml.

• 公益社団法人 電気化学会の論文

著者

• SHIKU Hitoshi

  Graduate School of Environmental Studies, Tohoku University

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• Cell-Based Electrochemical Assay for Endotoxin Using a Secreted Alkaline Phosphatase Reporter System

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