Preparation of Immunosensors Using a Microfluidic Device with an Interdigitated Array Electrode Modified with Antibodies
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概要
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We prepared a microfluidic device with an interdigitated array electrode modified with antibodies to develop immunosensors. A comb-type array (W1) was used for forming immunocomplexes and another (W2) for detecting mediators generated by the enzyme reaction. We used electropolymerization and avidin-biotin complexes for an addressable immobilization of antibodies on W1. Since the microfluidics significantly accelerated the formation of the immunocomplexes, a period as short as 10 min was sufficient to detect the responses. The current observed at bare array (W2) increased with increasing analyte (mouse IgG) concentration in the range of 1.0–100 ng/mL. Therefore, present procedure is suitable for rapid immunosensing in a simple device.
著者
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Yasukawa Tomoyuki
Graduate School Of Engineering Tohoku University
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Shiku Hitoshi
Graduate School Of Environmental Studies Tohoku University
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Mizutani Fumio
Graduate School Of Material Sci. Univ. Of Hyogo
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Matsue Tomokazu
Graduate School Of Environmental Studies Tohoku University
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HIRANO Yu
Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial, Science and Technology (AIST)
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SAWAYASHIKI Yoshihiro
Graduate School of Environmental Studies, Tohoku University
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