Cloning and Sequence Analysis of Endopolygalacturonase Genes in Venturia nashicola and Venturia pirina
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概要
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Genes encoding endopolygalacturonase (endoPG) were isolated from pathogens of the Asian pear scab, <I>Venturia nashicola </I>races 1, 2, 3, and 4, and European pear scab, <I>V. pirina</I>. The <I>Vnpgr1 </I>gene of the <I>V. nashicola </I>race 1 consists of a 1,116-bp open reading frame, encoding a protein of 372 amino acids with an estimated molecular mass of 37.5 kDa and an isoelectric point (pI) of 6.56. The sequences of <I>Vnpg </I>genes from different races and <I>Vppg </I>gene from <I>V. pirina </I>showed high identities (95-100%). The deduced amino acid sequence of the <I>V. nashicola </I>race 1 endoPG showed 63-68% identity to the endoPG sequences of <I>Penicillium olsonii, Colletotrichum lindemuthianum, Cryphonectria parasitica, Fusarium oxysporum, Sclerotinia sclerotiorum</I>, and <I>Alternaria citri</I>. The deduced amino acid sequence of the race 1 endoPG was identical to the N-terminal amino acid sequence of the previously purified endoPG enzyme from the mycelia of this race. The results of a southern blot analysis indicated that <I>V. nashicola </I>race 1 (isolate JS-115) had a single copy of the <I>Vnpgr1</I>. Restriction fragment length polymorphism (RFLP) analysis of the polymerase chain reaction (PCR) products of the endoPG gene digested with <I>Hinc</I>II, <I>Bsp</I>EI, and <I>Bsr</I>GI was performed; thereafter, agarose gel electrophoresis yielded race-specific RFLP patterns.
- 独立行政法人 国際農林水産業研究センターの論文
著者
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Ishii Hideo
National Inst. Agro‐environmental Sci. Ibaraki Jpn
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KATOH Hiroshi
National Institute for Agro-Environmental Sciences
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YAMADA Ayumi
National Institute for Agro-Environmental Sciences
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AKIMITSU Kazuya
United Graduate School and Faculty of Agriculture, Kagawa University
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