VHSVヒラメ由来株はニジマス稚魚に対し病原性を有する

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概要

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• We evaluated the pathogenicity of Korean and Japanese isolates of viral hemorrhagic septicemia virus (VHSV: genotype IVa) from olive flounder Paralichthys olivaceus using rainbow trout Oncorhynchus mykiss fry. The cumulative mortalities of fish challenged with FYeosu05 (Korean) and Obama25 (Japanese) isolates at 106.5 TCID50/fish were 64 and 48%, respectively. No mortality was observed among the fish challenged with either of the isolates at 105.5 TCID50/fish, or in mock-challenged fish. The affected fish exhibited darkening of the body, an expanded abdomen, pale gills, an enlarged spleen, and diffuse necrosis in the splenic and interstitial hematopoietic tissues. We re-isolated VHSV from all mortalities using cell culture and confirmed their presence by reverse transcription loop-mediated isothermal amplification (RT-LAMP). We thus concluded that Asian VHSV isolates from olive flounder are pathogenic to rainbow trout fry, although with low virulence.

著者

• JUNG SUNG-JU

  Department of Aqualife Medicine, Chonnam National University

• Nishizawa Toyohiko

  Department of Aqualife Medicine, Chonnam National University

• Jung Sung-ju

  Department Of Aqualife Medicine Chonnam National University

• Suebsing Rungkarn

  Faculty Of Marine Bioscience And Technology Gangneung-wonju National University

• Oh Myung-Joo

  Department of Aqualife Medicine, Chonnam National University

• Kim Wi-Sik

  The Fisheries Science Institute, Chonnam National University

• Kim Jeong-Ho

  Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University

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• VHSVヒラメ由来株はニジマス稚魚に対し病原性を有する
• VHSVヒラメ由来株はニジマス稚魚に対し病原性を有する
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