FGF-2 が細胞外基質グラフトによる腱板骨結合部の再構築に及ぼす影響

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Background: The purpose of this study was to investigate the histological and molecular biological effect of the local application of fibroblast growth factor (FGF)-2 on regeneration and remodeling of rotator cuff tendon defects reconstructed with acellular matrix bioscaffold (AMB) grafts in rats. Methods: Adult male Sprague-Dawley rats were assigned to equal groups of FGF-treated and untreated reconstructions. All rats underwent an AMB graft (GraftJacket, Wright med) for a supraspinatus defect (3 x 5 mm). FGF-2 (100 micro-g/kg) in a fibrin sealant was applied to both shoulders of the FGF-treated group, while only fibrin sealant was applied to the untreated group. Rats in each group were sacrificed at 2, 6, and 12 weeks after surgery for histological analysis using tendon-to-bone maturing scores, proliferating cell nuclear antigen (PCNA) assay, and quantitative real-time RT-PCR. Results: At 2 weeks, the FGF-treated group had similar tendon-to-bone maturing scores to the untreated group, but more PCNA positive cells and higher mRNA levels of col1, col3 and Biglycan (p<.05). At 6 and 12 weeks, the FGF-treated group had significantly higher tendon-to-bone maturing scores than the untreated group (p<.05) and similar mRNA levels of col1, col3 and Biglycan. Conclusion: The histological remodeling of AMB grafts placed in rat rotator cuff defects was accelerated by the local administration of FGF-2 due to increasing the number of proliferating cells and gene expression of col1, col3 and Biglycan in the graft. Histology and molecular biology in AMB graft constructs may be improved by the local administration of FGF-2 at the time of rotator cuff reconstruction.

FGF-2 が細胞外基質グラフトによる腱板骨結合部の再構築に及ぼす影響

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