Radiation Resistance of Spores of Clostridium botulinum Type E (I)

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The effect of some factors on the radiation resistance of spores of Clostridium botulinum type E was studied. The following resultswere obtained. (1) No significant difference was observed in the radiation resistance of the spores irradiated at 0, 10, and 20°C, It was, however, observed that the radiation death rate decreases with the increasing temperatures between 20°C and 50°C. At temperatures below-10°C, the radiation resistance of the spores increased again . The minimum point of the radiation resistance of the spores was found to be at around 0°C. (2) Organic and inorganic media were found to offer a slight protecting effect against radiation as compared with distilled water. (3) Spore suspensions irradiated at equivalent dose levels showed much, less viability with post irradiation incubation temperatures of 10 and 20°C as compared with of 30°C. (4) A total of 14 type E strains isolated in Hokkaido were tested for their .radiation resistance of spores. The average D-value was 0.138 Mrad, ranging from the maximum value of 0.174 Mrad to the minimum of 0.103 Mrad.
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