Effect of Gamma Irradiation on Fermentation Medium Consisting Mainly of Cane Molasses

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The applicability of radiopasteurization method to fermentation media consisted mainly of molasses was investigated. It was found that γ-radiation had an excellent pasteurization effect on the fermentation media and an increasing effect of ethanol fermentation rate and ethanol yield on the irradiated mashes. Per cent survivals in molasses decreased to ca. 70% with heating at 80°C for 30 min, toca, 10% with irradiation by 3.0×105R and to ca. 1% by 6.0×105R (Table 2). The irradiated mashes were suitable for media of the "starter", since the rate and the degree of the growth ofSaccharornyces cerevisiae in irradiated mashes did not differ from the growthin in heat-pasteurized mashes (Fig. 2). In case of the molasses mashes added nitrogen sources, ethanol fermentation rate and yield in irradiated mashes were larger than those in heated mashes (Figs. 3 and 4). Besides. a 14% difference was seen between fermentation yield in mashes irradiated by 3.0×105R and that in mashes heated at 80°C when no nitrogen sources were added to the mashes (Fig. 4). In the dose range between 1.0×105and9.5×105R the concentrations of total sugar and direct reducing sugar, pH (Fig. 5), and optical density of molasses were little affected by radiation.
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