Analysis of gamma irradiated pepper constituents. (Part 3). The supercritical fluid extraction of pepper perfume.:The Supercritical Fluid Extraction of Pepper Perfume

概要

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The extraction of pepper perfume by use of supercritical fluid extraction (SFE) was investigated. Carbon dioxidewas used as supercritical mobile phase. SFE was achieved by follow conditions, temperature was 40°C, pressurewas 200 kgf/cm<SUP>2</SUP>, 5% methanol was added to mobile phase and the extraction time was 60 minutes. The extractedfraction by this method was yellow oily substance and was pepper perfume rich fraction, while the residue of extraction had not only perfume but also any pungency. And it seems that most part of pepper perfume was extractedby Supercritical fluid extraction. The perfume fraction was analyzed by two way method, that is, supercriticalfluid chromatography (SFC) and reversed phase HPLC. SFC conditions were same as SFE. HPLC conditionswere described as the first report. By both methods, about 7 peaks were detected. According to their analisys ofthis fraction, the main component was identified as piperine. On the chromatogram of reversed phase HPLC of extract, most peaks were eluted later than piperine. And it is thought that most perfume components have high hydrophobicitymore than piperine. The change of perfume of pepper was able to be discussed based on the eaxtractionby SFE.
日本食品照射研究協議会の論文