Effect of Irradiation and Food Preservatives on the Keeping Quality of Fish Fillets:II.. Change in the microflora of irradiated and food preservative treated fish fillets during storageat 0°C
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The microfloral changes in irradiated (0.1 Mrad) and foodpreservative-treated northern halibut and big-eyed tunafillets during aerobic (packaged with thin polyethylene film), refrigerated storage at 0° were determined by the identificationof bacterial and yeast isolates to the generic level.As reported in the previous paper, two species of fish fillets were treated with either chlortetracycline (CTC, at 10 ppm), furylfuramide (FF, at 5 ppm), or tylosin (TL, 10 ppm). Half of which were subjected to irradiation at 0.1 Mrad of Co<SUP>60</SUP>γ Arays. All the samples being stored at 0° for 0, 3 and 6weeks were analyzed their microfloral composition according to the determinative schemes shown in Figures 1 and 2.<BR>The flora of pre-irradiated fresh fish fillets and those of non-irradiated-food preservative treated fillets are shown in Table 1 and 3 respectively. The great majority of survivingand growing organisms in the control and drug treated fillets during storage was <I>Pseadomonas, Aficrococci</I> and other Gram-positive organism in the CTC and FF treated samples, declined during storage.<BR>As can be seen in Table 2, Gram-positive organisms, especially yeast become predominant after irradiation with or without food preservatives among the microflora of halibut fillets especially the organisms become predominant in the flora of CTC or FF treated fillets during refrigerated storage.<BR>To the contrary, as can be seen in Table 4, the irradiated flora of tuna fillets of nontreated with drug consisted of <I>Enterobaoteriaceae, Micrococcus</I> and<I> Microbacterium-Corynebacterium</I>, and during low temperature storage, <I>Pseudomonas</I> and yeast were dominated. It was obvious that the pretreatment with either CTC or FF changed the microflora of surviving and growing organism in the test fillets during storage, however, as far as the present study is concerned it was rather difficult to evaluate the composition of microflora quantitatively due to shortage in number of the isolates.<BR>In Table 5, some characters <I>Psedomonas</I> isolates associating with putrofying activity was illustrated.
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