Development of Industrially Important .ALPHA.-Amylases.

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概要

• 論文の詳細を見る

• A new calcium independent α-amylase has been developed for starch liquefaction. This amylase Termamyl LCTM gives the same performance in the absence of free calcium compared to Termamyl<SUP>TM</SUP>, in the presence of 40 ppm free calcium. The improvement is a result of 5 single amino acid substitutions and one N-terminal alteration. Several new alkaline α-amylases were identified in Nature. We have isolated two amylases that display the desired activity profile but lack the overall stability necessary for a detergent a-amylase. A two amino acid deletion in the region from position 180-184 increased the overall stability both with respect to temperature and calcium sensitivity, to a satisfactory level comparable to that of Termamyl<SUP>TM</SUP>.

• 日本応用糖質科学会の論文

著者

• Borchert Torben

  Enzyme Business Novo Nordisk A/s

• Pedersen Sven

  Enzyme Business Novo Nordisk A/s

• Norman Barrie

  Enzyme Business Novo Nordisk A/s

• BISGAARD-FRANTZEN Henrik

  Enzyme Business Novo Nordisk A/S

• SVENDSEN Allan

  Enzyme Business Novo Nordisk A/S

• OUTTRUP Helle

  Enzyme Business Novo Nordisk A/S

• Kjaerulff Søren

  Enzyme Business Novo Nordisk A/S

関連論文

• Development of Industrially Important α-Amylases (糖質関連酵素化学シンポジウム)
• Development of Industrially Important .ALPHA.-Amylases.

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