A stopped-flow kinetic study of the solubilization of acridine orange and its 10-alkyl derivatives into the micelle of sodium dodecyl sulfate.

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概要

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• Stopped-flow method has been used to investigate the kinetics of the solubilization of Acridine Orange (AO, 3,6-bis(dimethylamino)acridinium chloride) and its 10-alkyl derivatives into the micelle of sodium dodecyl sulfate (SDS). The reaction curves of the SDS–AO and SDS–10-alkyl AO systems were characterized by single and double exponential functions, respectively. It was found that all the time constants are concentration dependent and approach limiting values at high reactant concentrations. The data were fitted by a minimal mechanism in which a rapid bimolecular step is followed by one (AO) or two (10-alkyl derivatives of AO) sequential isomerizations. All the rate constants determined for this mechanism markedly decrease with an increase in the length of the introduced alkyl chain. The equilibrium constants for the bimolecular and first isomerization steps do not highly depend on the size of the alkyl chain, while the equilibrium constant for the second isomerization step progressively increases with increasing it. The spectral changes with time indicate that the bound dye gradually migrates to more hydrophobic environment inside the micelle. These results lead to the conclusion that the steps in the proposed mechanism correspond to rapid adsorption of the dye to the micelle surface site, followed by reorientation of the dye and then migration of its alkyl chain into the hydrophobic region of the micelle.

• 公益社団法人 日本化学会の論文

著者

• Kubota Yukio

  Department Of Chemistry And Chemical Engineering Yamagata University

• Murakami Kiyofumi

  Department of Chemistry, Faculty of Education, Yamaguchi University

• Omura Naofumi

  Department of Chemistry, Faculty of Science, Yamaguchi University

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