Spreadability of Ovalbumin Monolayers at Air-water Interface

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概要

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• The spreading processes of ovalbumin have been studied quantitatively at air-water interface with either no additive or a small amount of hydrochloric acid for controlling subphase pH. Limiting area which is obtained by extrapolating force(<I>F</I>)-area(<I>A</I>) curve to <I>F</I>=0, increases with time, <I>t</I>, which has elapsed after the application of protein solution at the surface of subphase. Near the isoelectric point, the spreading processes are completed instantaneously to give the equilibrated value of limiting area which is found to be 0.83 m<SUP>2</SUP>/mg. The processes are much slower at both sides of the point, but the limiting area in equilibrium is found to be the same as in the case of isoelectric pH. It has also been shown that the processes are expressed in terms of two first-order reactions.

• 公益社団法人 日本化学会の論文

著者

• Ishii Toshio

  Department Of Chemistry Chiba Institute Of Technology

• Muramatsu Mitsuo

  Department of Chemistry Faculty of Science Tokyo Metropolitan University

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