Kinetic Studies of the Aggregation of Acridine Orange onto Poly-(α,L-glutamic acid)
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The aggregation process of acridine orange (AO) onto the helical poly-α,L-glutamic acid (PLGA) was studied by means of a stopped-flow and rapid-scan spectrophotometer. After the flow stop, a rapid spectral change was observed in which the α-band and the β-band fell, and the γ-band and the polymer band rose. A mechanism of the process was proposed by which after a rapid nucleative process, AO molecules are cooperatively bound to form a dimer and that then the dimer grows to the oligomer and to the polymer of AO which has the form of a super-helix around the core of the α-helix of PLGA.
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