血漿Aldosteroneと11-Deoxycorticosterone (DOC) の同時測定:-測定法ならびに正常者および各種内分泌性高血圧症患者における成績-
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Aldosterone and DOC can be measured by the present assay in a single extract of 1.0-2.0 ml of plasma. The method consists of extracting plasma with dichromethane, separating the aldosterone and DOC on a Sephadex LH-20 column, and quantitating each steroid by radioimmunoassay. This method was demonstrated to be sensitive, accurate, and precise. The values obtained were in agreement with those previously reported by the other investigators using different methods. <BR>In 15 normal subjects, the mean level of plasma aldosterone was 4.8 ± 3.4 SD ng/100 ml and the corresponding level of DOC was 8.9 ± 3.8 SD ng/100 ml. <BR>Fifteen patients with Cushing's syndrome were studied. In 10 patients with adrenocortical hyperplasia, the mean level of DOC (29.8 ± 11.1 SD ng/100ml) was significantly higher than normal (P<0.001), and the mean level of aldosterone (2.5 ± 2.0 SD ng/100 ml) was slightly lower than normal (P<0.01). The mean value for plasma DOC in the remaining 5 patients with adrenal adenoma was 20.6 ± 8.5 SD ng/100 ml and this was significantly higher than normal (P<0.001). But the mean value for plasma aldosterone (4.8 ± 2.0 SD ng/100 ml) was not significantly different from normal. In 2 patients with adrenal hyperplasia and in 2 with adrenal adenoma, plasma DOC and aldosterone levels returned to normal after treatment of Cushing's syndrome. <BR>Three patients with 17α-hydroxylase deficiency had clearly elevated plasma DOC levels and normal aldosterone levels. In these patients plasma DOC returned to normal after treatment with betamethasone. <BR>Five of 6 patients with primary aldosteronism had elevated plasma DOC levels and the mean of 33.4 ± 11.2 SD ng/100 ml was significantly greater than normal (P<0.001) The mean level of plasma aldosterone (31.7 ± 12.8 SD ng/100 ml) was also significantly higher than normal (P<0.001). In 2 patients, plasma DOC and aldosterone returned to normal after the adenoma was removed.
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- 血漿Aldosteroneと11-Deoxycorticosterone (DOC) の同時測定:-測定法ならびに正常者および各種内分泌性高血圧症患者における成績-