免疫活性及びレセプター活性からみた尿中hCG分子の多様性に関する研究
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概要
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A sensitive bioassay (Radio receptor assay : RRA) for urinary hCG which is independent of plasma half life and metabolism has been developed by utilizing the specific binding of <SUP>125</SUP>I-hCG to 150-2000g fraction of homogenates of the rat testis.<BR>The receptor and immunological activity (RRA/RIA) ratio of urinary hCG in pregnant and trophoblastic diseased women was examined.<BR>In addition, the urine of women with normal pregnancies was studied for quantitative and qualitative differences among hCG molecules, by gel filtration on a Sephadex G-100, and each fraction was measured by RRA and radioimmunoassay (RIA).<BR>1) The sensitivity of RRA was 4 ng/ml with a precision (lambda) of 0.03. The intraassay coefficient of variation and that for inter assay were 2-10% and 12.1% respectively. There was a complete lack of cross reaction with PRL, hCGa, hCGβ, and FSH; this indicated a high specificity of the RRA.<BR>2) Receptor reactive hCG in urine throughout pregnancy showed a major peak in the 10th week and declined, but remained fairly constant until the 31th week of pregnancy. The RRA/RIA ratio in urinary hCG of normal pregnancies was highest in the first trimester and lower in the second and third trimester. The average value throughout gestation was 2.04 ± 0.18 (SE), which was about 2 times greater than that (1.08 ± 0.11 (SE)) of trophoblastic diseases (p<0.01).<BR>3) The RRA/RIA ratio in the patients with trophoblastic tumors was highest in the hydatidiform mole and lowest in the chorioepithelioma.<BR>4) When the urine of normal pregnancies was gel filtrated on a Sephadex G-100, it was found that receptor active molecules of hCG were present in urine of the first and second trimester.<BR>These results indicated that the RRA/RIA ratio in urinary hCG was distinct in normal pregnancies from trophoblastic tumors and would contribute sufficiently to an early diagnosis and the treatment of successive trophoblastic disease in follow-ups of patients with hydatidiform mole.
- 日本内分泌学会の論文
著者
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猪野 博保
徳島大学医学部産科婦人科学教室
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奈賀 脩
徳島大学医学部 産科婦人科学教室
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櫛木 範夫
徳島大学医学部 産科婦人科学教室
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藤島 一典
徳島大学医学部 産科婦人科学教室
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木下 恒夫
徳島大学医学部 産科婦人科学教室
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猪野 博保
徳島大学医学部 産科婦人科学教室
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