カテコールアミンのラジオレセプターアッセイ:(1) <SUP>3</SUP>H-norepinephrineを用いての検討
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概要
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A radioreceptor assay for catecholamines (CA) has been established utilizing the microsomal fraction of bovine myocardium as CA receptors and <SUP>3</SUP>H-norepinephrine (<SUP>3</SUP>H-NA) as a ligand. The bound fraction was separated from the free fraction by filtration through milipore filters. The following results were obtained : <BR>1) The optimal amount of the microsomal fraction was 250μg/tube, when <SUP>3</SUP>H-NA of 12,000 c.p.m was used. The appropriate conditions for incubation were 2 hours at 37°C in a pH 7.4 sucrose solution.<BR>2) By this method, norepinephrine was detectable in the range from 5 to 100ng/tube.<BR>3) Various compounds with catechol nucleus showed a cross-reaction in this radioreceptor assay system.<BR>4) Alpha-adrenergic blocking agents inhibited the binding of <SUP>3</SUP>H-NA, whereas beta-blocking agents were ineffective.<BR>These results suggest that the microsomal fraction of bovine myocardium contains alpha-adrenergic receptors which bind the catechol nucleus of CA.
- 一般社団法人 日本内分泌学会の論文