中枢内aromataseの制御機構 (第1報):視床下部aromatase活性のandrogen投与による影響

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Recently, some studies have found the greatest aromatase activity in brain areas associated with sexual differention and sexual behavior, namely the hypothalamic and limbic structures. We studied the regulation of aromatase activity in the hypothalamic area of male rats, using a sensitive in vitro assay which measures the amount of 3 H2 O formed by tissue homogenates during the conversion of [1β-3 H] androstenedione to estrogen. After castration, hypothalamic aromatase activity was significantly decreased (P<0.01), and seminal vesicle (SV) and prostate (PR) weights were also significantly decreased (P<0.01). Castrated male rats were given testosterone (T), 5α-dihydrotestosterone (DHT), 5α-androstane-3α, 17β-diol (A3α), 5α-androstane-3β and 17β-diol (A3β) in various doses (200-1000μg/day) for 10 days, and were given 600μg/day T, DHT, A3α and A3β for various durations (1-10 days). We found that T, DHT and A3α but not A3β reversed the effects of castration on the hypothalamic aromatase activity. The order of this reversible effect of androgens was as follows : T≥DHT>A3α. T, DHT, A3α and A3β increased SV and PR weights, and the order of this effect was as follows : DHT>T>A3α>>A3β. We administered the antiandrogen (flutamide) to intact male rats (8mg/day for 6 days). Flutamide decreased hypothalamic aromatase activity at the same level as that of castrated rats. Likewise, administration of both flutamide and T to castrated rats blocked the T-induced increase in hypothalamic aromatase activity and accessory sexual organ weight. From these results, we suggest that T, DHT and A3α regulated hypothalamic aromatase activity, that T was the most effective of the androgens, and that was different from peripheral androgen target organs.
一般社団法人日本内分泌学会の論文

中枢内aromataseの制御機構 (第1報):視床下部aromatase活性のandrogen投与による影響

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