蛍光眼底造影剤インドシアニングリーンのジミリストイルフォスファチジルコリンリポソームとの結合反応の分光学的研究

概要

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Indocyanine green (ICG) is a fluorescent dye that has been used as a reagent to examine liver function and for imaging of the retina and choroidal vasculatures. The binding reaction of ICG with Dimyristoylphosphatidylcholine (DMPC) liposome as a model of the cell membrane was examined using a spectroscopic technique. The DMPC liposome was prepared in the unilamellar condition and the binding of ICG was evaluated utilizing a metachromasia phenomenon. It was found that the binding reaction of ICG to the DMPC liposome proceeds stoichiometrically and greatly differs at lower and higher temperatures compared to the phase transition temperature of the membrane. Corresponding to the change of the membrane state, binding of the ICG molecule to the liposome becomes unstable and the binding constant decreases with temperature. Near the phase transition temperature, the binding constant was at a minimum. It is suggested that the membrane fluidity greatly influences the binding properties of the ICG molecule.
日本バイオレオロジ-学会の論文