遠心限外濾過法によるAndrogenの血清蛋白結合に関する研究:特に, Dehydroepiandrosterone sodium sulfateのウシ血清蛋白との結合について

スポンサーリンク

概要

• 論文の詳細を見る

• Studies were carried out for the elucidation of the binding of dehydroepiandrosterone sodium sulfate (DHA-S) with bovine serum protein by the method of centrifugal ultrafiltration. In order to examine the stoichiometric relation among the concentrations of DHA-S, protein, and DHA-S protein complex, twoseries of experiments were performed.<BR>1) With the constant concentration of protein (3.4 gm/dl), the concentration of DHA-S was varied, i.e., 80,160,240,320, and 400μg/cc.<BR>2) With the constant concentration of DHA-S (400, μg/cc) the concentration of bovine serum protein was varied, i.e., 1.7, 3.4, 5.1 and 6.1 gm/dl.<BR>These solutions were equilibrated with 5% CO<SUB>2</SUB> in air for 20 to 30 minutes at 37°C (pH 7.4) and 5cc of the solution was centrifuged for 15 minutes at 3000 r.p.m., the radius of rotation being 15cm. 1 cc of ultrafiltrate was acid hydrolyzed and extracted with ether. The colorimetric determination of DHA was carried out according to the method of Zimmermann.<BR>The results were as follows : <BR>1) The concentration of diffusible DHA-S in ultrafiltrate was 13.7, 17.0, 31.5, 44.6, 62.8 μg/gmH<SUB>2</SUB>O when the original solution contained DHA-S at the concentration of 80,160,240,320, and 400 μg/cc respectively with the constant concentration of protein.<BR>2) In this series of experiments the ratio of the amount of the bound DHA-S to the total amount of DHA-S added was 83.5, 89.8, 87.1, 86.6, and 84.9% and there seems to be no significant difference among these values. The amount of bound DHA-S per unit amount of protein was 2.1, 4.4, 6.5, 8.5, and 10.2 mg/gm protein respectively.<BR>3) In the second series of experiments where the protein concentration in the original solution was changed keeping the total concentration of DHA-S constant, the concentration of diffusible DHA-S in the ultrafiltrate varied in reciprocal fashion. To the protein concentration of 1.7, 3.4, 5.1, and 6.1 gm/dl the corresponding values of diffusible DHA-S obtained were 111.3, 46.9, 17.5, and 12.2 μg/gmH<SUB>2</SUB>O.<BR>4) The ratio of the bound steroid to the total amount added increased as the protein concentration increased, the corresponding values being 72.1, 88.3, 95.6, and 97.0%. The amount of the bound DHA-S was 16.0, 9.9, 7.1, and 6.0 mg/gm protein.<BR>5) These results were analyzed according to the equations of Freundlich and of Klotz. The data seem more suitable for the equation of Freundlich so that the binding of DHA-S with serum protein seems to be due to physical adsorption.

• 一般社団法人 日本内分泌学会の論文

著者

• 石川 久夫

  金沢大学医学部産科婦人科

• 石川 久夫

  金沢大学医学部産科婦人科学教室

関連論文

• 3. 当教室における子宮癌の細胞診の成果について(A.婦人***に関する問題, I.一般講演, 第7回日本臨床細胞学会講演要旨)
• 45. 女子尿中17-KS値の年齢的推移,とくに卵巣との関連性について
• 当教室における子宮癌の細胞診の成果について
• 産科婦人科学領域における老年医学的研究 : とくに閉経後婦人における副腎性androgenの意義と応用
• 51. 悪性腫瘍患者の網内系機能状態について(特に流血中リンパ球数との関連に於て)
• 36. 頸癌患者に於ける尿中男女性ホルモンの動的比較検討
• 36. 頸癌患者に於ける尿中男女性ホルモンの動的比較検討
• 遠心限外濾過法によるAndrogenの血清蛋白結合に関する研究:特に, Dehydroepiandrosterone sodium sulfateのウシ血清蛋白との結合について

スポンサーリンク