Effect of Matrigel on the Osteogenic Potential of Canine Adipose Tissue-Derived Mesenchymal Stem Cells
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概要
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Adipose tissue-derived mesenchymal stem cells (Ad-MSCs) are a promising source of cells for bone tissue engineering. Matrigel is a basement membrane extract containing multiple extracellular components. This mixture may promote the osteogenic differentiation of MSCs and provide a more appropriate microenvironment for transplanted cells. Here, we investigated the effect of Matrigel on the osteogenic potential of Ad-MSCs. Canine Ad-MSCs were cultured in 2D and 3D matrices and implanted into subcutaneous pouches of dogs either with or without Matrigel. Culture mineralization, cell adhesion efficiency, cell proliferation, osteoid matrix production and alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase activities were quantified and compared. Ad-MSCs grown in 2D cultures with Matrigel showed higher levels of calcium deposition and ALP activity than those grown in the absence of Matrigel under osteogenic conditions. In 3D cultures, the cells cultivated with Matrigel showed greater attachment, proliferation and osteogenic differentiation than those grown without Matrigel. In vivo, Ad-MSCs implanted with Matrigel showed higher osteogenic potential than those without Matrigel. In conclusion, these data suggest that the use of Matrigel can increase the osteogenic potential of canine Ad-MSCs.
著者
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Kweon Oh-kyeong
Department Of Veterinary Surgery College Of Veterinary Medicine Seoul National University
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Park Sung-su
Department Of Veterinary Surgery College Of Veterinary Medicine Seoul National University
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Kim Wan
Department Of Microbiology College Of Natural Science Pusan National University
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RYU Hak-Hyun
Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Daehak-dong, Gwanak-gu, Seoul 151–742, Korea
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KWEON Oh-Kyeong
Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Daehak-dong, Gwanak-gu, Seoul 151–742, Korea
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KIM Wan
Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Daehak-dong, Gwanak-gu, Seoul 151–742, Korea
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KANG Byung-Jae
Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Daehak-dong, Gwanak-gu, Seoul 151–742, Korea
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PARK Sung-Su
Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Daehak-dong, Gwanak-gu, Seoul 151–742, Korea
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KIM Yongsun
Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Daehak-dong, Gwanak-gu, Seoul 151–742, Korea
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WOO Heung-Myong
College of Veterinary Medicine and KNU Stem Cell Institute, Kangwon National University, Chuncheon 200–701, Korea
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WOO Heung-Myong
College of Veterinary Medicine and KNU Stem Cell Institute, Kangwon National University, Chuncheon 200–701, Korea
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