Identification of Novel Immunogenic Proteins in Mycoplasma capricolum subsp. Capripneumoniae Strain M1601
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概要
- 論文の詳細を見る
The purpose of this study was to obtain immunogenic proteins and potential proteins of interest that were isolated from Mycoplasma capricolum subsp. capripneumoniae (Mccp) by MALDI-TOF mass spectrometry. One-dimensional SDS-PAGE and two-dimensional gel electrophoresis of whole cell preparation were conducted, and membrane proteome maps were prepared by immunoblotting. One-dimensional SDS-PAGE identified three immunogenic proteins with molecular masses in the range 29–97.2 kDa, two of which were in the membrane protein fraction. After two-dimensional gel electrophoresis, 20 highly immunogenic proteins were identified in the whole cell protein preparation while 9 immunogenic proteins were identified in the membrane protein fraction. This indicated that membrane proteins were the principle immunogenic proteins in Mccp. These proteins may have potential for the development of improved diagnostic tests and possible vaccines.
- 日本獣医学会の論文
著者
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Zhang Xuan
State Key Laboratory Of Natural And Biomimetic Drugs And School Of Pharmaceutical Sciences Peking Un
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ZHAO Ping
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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HE Ying
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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CHU Yue-feng
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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GAO Peng-cheng
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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ZHANG Nian-zhang
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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ZHAO Hai-yan
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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ZHANG Ke-shan
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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LU Zhong-xin
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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ZHAO Hai-Yan
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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CHU Yue-Feng
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animal of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
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CHU Yue-Feng
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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ZHAO Ping
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animal of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
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ZHAO Ping
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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GAO Peng-Cheng
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animal of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
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GAO Peng-Cheng
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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ZHANG Xuan
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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LU Zhong-Xin
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animal of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
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LU Zhong-Xin
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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ZHANG Ke-Shan
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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HE Ying
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animal of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
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HE Ying
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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ZHANG Nian-Zhang
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animal of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
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ZHANG Nian-Zhang
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological De
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- Identification of Novel Immunogenic Proteins in Mycoplasma capricolum subsp. Capripneumoniae Strain M1601