Extract from Nandina domestica Inhibits Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in Human Pulmonary Epithelial A549 Cells

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概要

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• Extract from fruits of Nandina domestica THUNBERG (NDE) has been used to improve cough and breathing difficulty in Japan for many years. To explore whether NDE may alleviate respiratory inflammation, we investigated its effect on expression of cyclooxygenase-2 (COX-2) and production of prostaglandin E2 (PGE2) in human pulmonary epithelial A549 cells in culture. Treatment with lipopolysaccharide (LPS; 6 µg/mL) resulted in an increase of COX-2 expression and PGE2 production in A549 cells. Both the LPS-induced COX-2 expression and PGE2 production were significantly inhibited by NDE (1–10 µg/mL) in a concentration-dependent manner. NDE did not affect COX-1 expression nor COX activity. These results suggest that NDE downregulates LPS-induced COX-2 expression and inhibits PGE2 production in pulmonary epithelial cells. Furthermore, higenamine and nantenine, two major constituents responsible for tracheal relaxing effect of NDE, did not mimic the inhibitory effect of NDE on LPS-induced COX-2 expression in A549 cells. To identify active constituent(s) of NDE responsible for the anti-inflammatory effect, NDE was introduced in a polyaromatic absorbent resin column and stepwise eluted to yield water fraction, 20% methanol fraction, 40% methanol fraction, 99.8% methanol fraction, and 99.5% acetone fraction. However, none of these five fractions alone inhibited LPS-induced COX-2 expression. On the other hand, exclusion of water fraction from NDE abolished the inhibitory effect of NDE on LPS-induced COX-2 expression. These results suggest that constituent(s) present in water fraction is required but not sufficient for the anti-inflammatory activity of NDE, which may result from interactions among multiple constituents.

著者

• Abe Kazuho

  Laboratory Of Chemical Pharmacology Graduate School Of Pharmaceutical Science The University Of Toky

• Akaishi Tatsuhiro

  Laboratory Of Pharmacology Faculty Of Pharmacy And Research Institute Of Pharmaceutical Sciences Mus

• Okumura Hidenobu

  Tokiwa Pharmaceutical Co. Ltd.

• Ueki Takuro

  Laboratory Of Pharmacology Faculty Of Pharmacy And Research Institute Of Pharmaceutical Sciences Mus

• ABE Kazuho

  Laboratory of Pharmacology, Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University

• Ueki Takuro

  Laboratory of Pharmacology, Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University

• Akaishi Tatsuhiro

  Laboratory of Pharmacology, Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University

• Okumura Hidenobu

  TOKIWA Pharmaceutical Co., Ltd.

関連論文

• Biphasic Tracheal Relaxation Induced by Higenamine and Nantenine From Nandina domestica THUNBERG
• The Extract from Nandina domestica THUNBERG Inhibits Histamine- and Serotonin-Induced Contraction in Isolated Guinea Pig Trachea(Pharmacology)
• Measurement of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) reduction activity and lactate dehydrogenase release using MTT
• Biphasic Tracheal Relaxation Induced by Higenamine and Nantenine From Nandina domestica THUNBERG
• Extract from Nandina domestica Inhibits Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in Human Pulmonary Epithelial A549 Cells
• Extract from Nandina domestica Inhibits Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in Human Pulmonary Epithelial A549 Cells

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