妊娠後期,授乳期,授乳停止後におけるマウス乳腺の電顕組織化学的研究〔英文〕
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The periodic acid-chromic acid-silver methenamine (PA-CrA-silver) method and the chromic acid-phosphotungstic acid (Chromic acid-PTA) method were applied to the mouse mammary gland cells for the ultrastructural observations of the synthetic process of the secretory substances and the secretory mechanism of secretory granules in the late stage of pregnancy, lactating and postlactating periods, and the relationship between lysosomes and secretory granules during postlactating periods. Golgi apparatus in the mammary gland cells of pregnant and lactating mice with active secretion showed the gradient staining from cis side to trans side in both the PA-CrA-silver method and the Chromic acid-PTA method. The positive reaction products of the complex carbohydrates were recognized in the clear spaces of casein granules and multicasein bodies, but the cores showed negative reaction. The surfaces of microvilli and the lysosome showed positive reaction while fat droplets showed negative reaction. The cisternae of rough endoplasmic reticulum were weakly stained in PA-CrA-silver methenamine, but not stained in Chromic acid-PTA. The positive reaction of rough endoplasmic reticulum (RER) in the PA-CrA-silver reaction was easily observed in the pregnant and lactating periods, when the secretory mechanism became active. These findings suggest that some glycosylation occurs in RER in the mammary gland cells.<BR>On the 4th day of the postlactating period, the innermost element of Golgi cisternae as well as Golgi associated endoplasmic reticulum lysosome system (GERL) showed intense acid phosphatase activities, whereas the staining of PA-CrA-silver as well as Chromic acid-PTA was greatly reduced in the Golgi cisternae. On the 8th day of the postlactating period, the primary lysosome was decreased but the secondary lysosome and autophagic vacuole were increased in the cytoplasm of mammary gland cells. This phenomenon indicates that the excessive casein granules are digested by primary lysosomes during postlactating periods.
- 学校法人 昭和大学・昭和医学会の論文
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