Acid α-naphtyl acetate esterase(ANAF)染色法による皮膚組織内浸潤細胞のT・B cell typeの解析ならびに若干の皮膚疾患における所見
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Lymphocyte in the cutaneous infiltration was studied with acid α-naphtyl acetate esterase (ANAE) staining method (Horwitz et al.) using paraphin embeded specimen. Materials were 30 (33 Specimens) of verruca senilis, 22 of benign diseases and 20 of malignant tumors.<BR>The results obtained were as follows : 1. As preliminary examination, the staining was applied to peripheral blood smear preparations and T, T′, B.and M patterns were confirmed by staining behabier. 2. It was then confirmed that E-rosette forming lymphocyte revealed T pattern and EAC-rosette forming lymphocyte B pattern. 3. As for 33 specimens of verruca senilis, the majority of lymphocyte revealed T pattern among 12 of hyperkeratotic type and 3 of irritated seborrheic keratosis. However, the majority of lymphocyte revealed B pattern among 16 of acanthotic type and 2 of adenoid type. 4. Almost all cases of malignant tumors showed T pattern predominance. On the specimens of cutaneous T-cell lymphoma, the great number of lymphocyte in the vicinity of epidermis showed T pattern befor treatment. However, after treatment, almost all of lymphocyte had been disappeared and remnant lymphocyte showed B pattern. 5. The majority of lymphocyte showed B pattern predominance among ? 2 of benign diseases. But lupus miliaris disseminatus f aciei showed T pattern predominance. It has been reported that not all the lymphocytes showed T pattern, but only helper T cell showed T pattern and there were false positive, thus lowerer number of T cell than actual number of them would be indicated. However, this study showed that the method had the advantage of the short time of reaction and the staining result was excellent. The method are not so available in Japan, but the method is useful to observe the distribution and localization of T and B cell in the cutaneous infiltration in situ.
- 昭和大学・昭和医学会の論文
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- Acid α-naphtyl acetate esterase(ANAF)染色法による皮膚組織内浸潤細胞のT・B cell typeの解析ならびに若干の皮膚疾患における所見