Thymoxamineの肝に及ぼす影響-3-赤血球膜作用とラット単離肝細胞に及ぼす影響について

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In this study, we investigated the effects of thymoxamine (Mox) and its metabolites, deacetyl-thymoxamine (DAM) and deacetyl-demethyl-thymoxamine (Met-X) and ifenprodil (Ifen) on isolated rat hepatocytes and erythrocytes. The hepatotoxicity of various drugs (Mox, DAM, Met-X, Ifen and phentolamine) to isolated rat hepatocytes was determined by the leakage of GPT, GOT and LDH into the assay media. Inhibition of the hypotonic hemolysis of rat erythrocytes was measured by hemoglobin concentrations. Mox, DAM and Met-X (1 × 10-6-1 × 10-3M) showed no membrane effects on erythrocytes and isolated hepatocytes. Ifen showed both a slight membrane stabilizing effect on erythrocytes in lower concentration and hemolytic effect in higher concentration (2.5 × 10-4-1 × 10-3M) . Ifen (1×10-3M) caused GPT and LDH leakage from hepatocytes. These results showed that the effects of Ifen on cell membrane are greater than those of Mox, DAM and Met-X. It seems that Mox and its metabolites, DAM and Met-X do not produce direct hepatic cell damage, based on our findings.
学校法人昭和大学・昭和医学会の論文