常在菌感作による実験的過敏性肺蔵炎

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Experimental allergic interstitial pneumonitis was induced in rabbits received the prolonged sensitization, the intrapulmonary injection and the transtracheal inhalation by using Klebsiella pneumoniae (K) and Clostridium perfrigens (Cl) .<BR>The prolonged sensitization was performed by the injection of the killed bacteria, cell wall or supernatant fraction, which were emulsified in incomplete Freund's adjuvant, once a week for 13 to 27 weeks. The pulmonary specimens revealed marked interstitial damage, characterized by the formation of granuloma, the infiltration of mononuclear cells and the appearance of giant cells. By immunofluorescent study, rabbit IgG was found in the interstitial granulomatous tissue. The severer lesion was found in the group of rabbits injected with cell wall fraction than the other fractions.<BR>The intrapulmonary injections with these bacterial antigens was performed in rabbits, which have pre-sensitized with the antigens 14 days before. The pulmonary tissue directly injected with homologous (K-sensitized→K-injected, Cl-sensitized→Cl-injected) or heterologous (K→Cl, CI→K) antigens, revealed the interstitial granulomatous pneumonitis, predominantly in the surrounding area of the injected site. The severer pulmonary lesion was found in the groups of rabbits injected with homologous antigen than with the heterologous antigen.<BR>The experiment of transtracheal inhalation by using the bacterial antigens was performed in the groups of rabbits which have received one shot with the bacterial antigen 14 days before or 4 times of the injections once a week. The rabbits were sacrificed 3, 12, 48 hrs. and 7 days after the inhalation. The group of rabbits which have received one shot injection showed delayed type reaction by skin test and no or only weak precipitating antibody against the injected antigen. The pulmonary lesion revealed the interstitial granulomatous pneumonitis, characterized by infiltration of mononuclear cells (predominantly) and polymorphonuclear leucocytes in the alveolar septa and space. The lesion became predominant 12 to 48 hrs. after the inhalation. The group of rabbits received the multiple (4 times) injection showed Arthus type reaction in skin test and were detected precipitating antibody. The pulmonary lesion revealed also the interstitial pneumonitis, characterized with the infiration of polymor-phonuclear leucocytes in alveolar septa in 3 hrs. after the inhalation, thereafter the infiltration of mononuclear cells became prominent. The severity of the pneumonitis was correlated wtih the titer of precipitating antibody. By immunofluorescent study, antigen of K. and Cl. were detected in the pulmonary interstitial, presumably in macrophage as early as 3 hrs. and also rabbit IgG were observed in granulomatous lesion and bronchial mucous.<BR>These data suggested that the bacterial antigen could induce the pulmonary lesion mediated by Arthus type reaction and/or delayed type reaction.
学校法人昭和大学・昭和医学会の論文

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小林玄彦
昭和大学医学部第一内科学教室

常在菌感作による実験的過敏性肺蔵炎

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