耐性ブドウ球菌感染症に関する研究
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概要
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Of all the factors to treat Pc resistent staphylococcal infection, it was considered to be most important to find out the action of, Pc in living bodies and the effect of combined use of various antibiotics in vitro and in vivo. The following experiments were performed and results were obtained.<BR>1) Inducing experimental pneumonia with Pc resistent staphylococci in rabbits, Pc-G was administered and its concentration in the blood, in the healthy and affected areas of the lungs were measured, one, two, three and five hours after the administration successively. The concentration was compared with those obtained in pneumonia with 209P strain.<BR>As a result, it was found that there was no significant difference of the concentration in blood and healthy areas of the lung between the both groups. On the other hand, the PC concentration in the involved areas of the lung with PC resistent staphylococci was found very low. However, the PC concentration in the involved areas of the lung with 209P strain was higher than those of blood. It was considered that the action of PC was significantly enfeebled in the affected area.<BR>2) Four hours after Pc administration, pneumococcemia was produced in 20% of the rabbits with the mixed use of Diplococcus pneumoniae and PC sensitive 209P strain, while it was produced approximately 50% with the mixed use of Diplococcus pneumoniae and PC resistant staphylococci. Thus there was a distinct difference between these two groups. However, no significance was observed twenty-four hours after PC administration when the PC concentration in the blood is supposed to fall to zero.<BR>3) When Cook strain of streptococcus haemolyticus and PC resistent staphylococci were buried in the abdominal cavity in agar column, the activity of PC on streptococcus haemolyticus was inhibited due to the PC resistant staphylococcus strain which had been mixed.<BR>4) Staphylococci, 209 strain and the strains, which were resistant to PC, SM, CP, TC, KM, EM and OM, were prepared and utilized in this experiment. Twenty-eight combined sets were made by combining two out of fight antibiotics and their effect were examined on each of the antibiotic resistant strains above discribed. Combined antibiotics showed a synergistic effect in most cases, while antagonistic effect in a few cases. However, it was not found that a certain combina-tion of antibiotics always acted synergistically or antagonistically on all the strains.<BR>5) Serum protein conjugation rate was examined by cellophane bag when PC was present together with SM and also with TC. It was found that there was no great difference in the serum protein conjugation rate between these cases and those in which each drug was used independently.<BR>6) PE-PC and CP were given to healthy men separately or combined with half amount each. The antibacterial activity in the blood was somewhat lower than the expected value, or the avarage value when they were given together. Therefore it was considered that further study should be performed.<BR>7) As in-vivo study, an experimental bacteriemia secondary to lobar pneumonia was induced in rabbits and a mixture of PC with CP or with TC were administered. No synergistic or antagonistic action was observed in the combination above mentioned.
- 学校法人 昭和大学・昭和医学会の論文