ラット脳の低酸素症における変化 特にP32摂取を中心として
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It is regarded that brain is easily affected by anoxia and various clinical events of neurology and psychiatry are basically connected with the problems of anoxia.<BR>Rat was ligated in right carotid artery and was exposed to nitrous oxide for one hour after the procedure of Levine to result in anoxic-ischemic encephalopathy. P-32 was administered intraperitoneally one hour prior to sacrifice. Brain was excised quickly and divided in bilateral hemispheres. After homogenizing water content, methylene blue reduction activity (M.B.R.A.) and P-32 uptake of each halves were measured.<BR>Increased water content, depressed M.B.R.A. and increased P-32 uptake were observed in the ligated, compared to unligated side, which might suggest the development of cerebral edema supression of aerobic glycolysis and increased permeability of blood-brain barrier to phosphate in ratbrain under to phosphate in rat-brain under the condition of anoxia. P-32 incorporated in the affected side mostly remained in acid-solubee fraction, not in acid-insoluble fraction. These cohanges in anoxia of rat-brain were studied with the lapse of time. M. B. R.A. in the affected side was depressed immediately after the hypoxia ; the increased P-32 uptake and the increased water content did not become apparent for a while. In the early period (6-9 hours elapsed after the hypoxia) these changes were so marked to be regarded as the hydrated or edematous phase. ; in the late period (around hours) they recovered. The water content and the P-32 uptake passed the time of light decreare in the late period as the dehydrated phase.<BR>M.B.R.A, and P-32 uptake hat the close linear relationship to water content, especially in the hydrated phase. These results seemed to mean the course of the supression of aerobic glycolysis, the development of cerebral edema and the increased permeability of blood-brain barrier induced by anoxia.<BR>These results were discussed on cerebral edema, blood-brain barrier and energy metabolism of brain. Further evidences of the changes in the experimental hypoxia of rat brain will be added in my future study.
- 学校法人 昭和大学・昭和医学会の論文