炭酸に関する研究
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The authors injected CO<SUB>2</SUB> saturated blood Ringer's solution into the ear vein of rab bits, whereby investigated the changes in the value of cholinesterase in the brain.<BR>According to the results of the experiment by Sekiya, the carbonic acid content in the blood in Vol. increases in 15-75 minutes after the injection, and roughly recovers the normal value after 5 hours. By this reason, the authors bled the rabbits to death at the time when the carbonic acid content in the blood reached approximately the maximum, i. e., 30 minutes after the injection, and the brains were immediately taken out to estimate the cholinesterase concentration in the brain. First of all, the authors bled the rabbits 15 minutes and 30 minutes after the injection by heart puncture, and estimated the carbonic acid content in the blood with Van Slyke's manometer. Generally, the values after 30 minutes thus obtained were higher than those obtained after 15 minutes. Further, in the case of the injection of either 3 cc or 5 cc of CO<SUB>2</SUB> saturated blood Ringer's solution, no remarkable changes were observed in the general conditions of the rabbits.<BR>On the other hand, as to the method of estimation of the cholinesterase in the brain, biological method of estimation or Ammon's method etc. were being employed in the past. The authors, however, employed a new colorimetric estimation method roughly according to Miyazaki and Ohara et al for the experiments. As the results of the comparison of the values of the estimation of cholinesterase in various animal tissues and in the serum by means of this method with those obtained by means of Ammon's method, Konno confirmed that the new colorimetric estimation method is simpler in the procedures and that its sensitiveness is higher compared with other method hitherto employed.<BR>The cholinesterase content in the brain is indicated in term of the amount of acetylcholin splitted in mg/30<SUP>m</SUP> 38°C for 0.16 g of brain tissue at 0.0125 mol of substrate. In the normal rabbits, the values were 1.39 mg, 1.08 mg, 1.48 mg, 1.46 mg and 1.22 mg, while in the cases of the rabbits injected with CO<SUB>2</SUB> saturated blood Ringer's solution, a tendency of slight increase was recognized. In the cases of the injections of 3 cc, the values were 1.58 mg, 1.52 mg, 1.96 mg, 1.83 mg and 1.71 mg, and in the cases of the injections of 5 cc, the values were 1.59 mg, 1.45 mg, 1.60 mg, 1.39 mg and 1.63 mg. Furthermore, in all of the cases, no evident correlationships were recognized between the weight of brain tissue and the room temperature.<BR>In short, although no definite could be drawn owing to the smallness in the number of the experimental cases, the cholinesterase content in the brain is considered slightly increased compared with that of the normal rabbits by the injection of the CO<SUB>2</SUB> saturated blood Ringer's solution.
- 学校法人 昭和大学・昭和医学会の論文