Morphological, electro-physiological and histochemical study of the cupula and the subcupular space.
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The cupula and the subcupular space were studied by scanning electron microscope (SEM) in ginea pigs, pigeons and chicks. Some ampullae were fractured by a styren lesion for observation of the relationship between the sensory hairs and the cupula. Electronmicroscopy revealed that the side of the cupula consists of a tubular structure. There were a number of small holes in the top and the bottom of the Cupula. On the surface of the crista were observed many small granules secreted by the supporting cells. Many sensory hair bundles traversed the subcupular space and were in contact with the cupular base.In a physiological study, the cupula of the Rana catesbiana was stimulated either by direct pressure or by mechanical endolymphatic flow. Action potentials from the posterior ampullary nerve were recorded before and after removal of the cupula and the subcupular substance. After cupular removal no action potential was evoked. When the cupula was replaced in the crista, the maximum frequency of the action potential in response to both direct pressure and mechanical endolymphatic flow recovered. However, the time course of the response was shorter than that prior to cupular removal.The cupula and the subcupular space were analyzed histochemically by light and electron microscopy. The ampulla of the chick was stained by periodic acid-Schiff (PAS), diastase-PAS, alcian blue-hyaluronidase, AB (pH2.5), AB (pH1.0), HID and AB-sialidase. The cupula and the intracellular microglomerus of the supporting cells contained mainly sialoglycoprotein (sialomucin). The cupula and the subcupular space were studied by both secondary electron (SE) image and backscattered electron (BSE) image. Ruthenium red (RR) staining was used for SEM observation of BSE image. The cupula and the granules of the crista surface contained mainly acid glyconjugate. Thus, the cupula and the gelatinous substance in the subcupular space contained mainly sialoglycoprotein.
- 耳鼻咽喉科臨床学会の論文
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- Morphological, electro-physiological and histochemical study of the cupula and the subcupular space.