THE DETERMINATION OF VITAMIN B12 ACTIVITY IN HUMAN BLOOD

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概要

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• The procedures for releasing and extracting vitamin B12 from proteins in whole blood was investigated by the microbiological assay using Lactobacillus leichmannii ATCC 4797.The treatment of the whole blood by heating with potassium cyanide was found to give constant values at each level of the vitamin, and good recoveries of the added vitamin in the assay.The individual values of the vitamin in whole blood estimated by heating with potassium cyanide are in agreement with those obtained by using sodium metabisulfite and those by papain digestion, but not with those by digestion with the trypsin preparation available in this country.The vitamin B12 content of healthy human whole blood was found to range from 0.10 to 0.66mγ/ml, the average value being 0.28±0.05 (U.S.A.) and 0.37±0.07mγ/ml (Japan).

• 日本ビタミン学会の論文

著者

• Murata Kiku

  Food And Nutrition Laboratories Teikoku Women's University

• MURATA KIKU

  Food and Nutrition Laboratory, Faculty of Science of Living, Osaka City University

• MIYAMOTO TEIJIRO

  Food and Nutrition Laboratory, Faculty of Science of Living, Osaka City University

関連論文

• Efficiency of Exogenous Quinolinic Acid as Niacin in Rats(Food & Nutrition)
• THE DETERMINATION OF VITAMIN B12 ACTIVITY IN HUMAN BLOOD
• THE PYRIMIDINE PART OF THIAMINE DECOMPOSITION PRODUCT BY BACTERIAL THIAMINASE I
• Comparison of the Activity of the Tryptophan-NAD Pathway between Rats Fed a Fat-free Diet and a Fat Diet
• Effects of an Intraperitoneal Injection of a Large Amount of Nicotinamide on NAD and Thiamine in Livers of Thiamine-deficient Mice

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