THE PYRIMIDINE PART OF THIAMINE DECOMPOSITION PRODUCT BY BACTERIAL THIAMINASE I

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概要

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• Although the detection of Dragendorff-positive spot on paper chromatogram due to the pyrimidine compound formed from thiamine by bacterial thiaminase I had failed thus far, it was shown in the present experiment that the crude crystalline pyrimidine compound (Pm-X⋅HCl) isolated from the thiamine reaction products by the bacterial thiaminase I gave a Dragendorff-positive spot at RF 0.06 in contrast with RF 0.59 of thiamine, when n-butanol methanol-water (4:2:2) was used as the solvent system for the development of paper chromatography.The absorption maximum of Pm-X·HCl extracted by 0.001N hydrochloric acid from a paper strip around RF 0.06 of the paper chromatogram was found at 250mμ, and the form of the absorption spectrum of the substance differed somewhat from that of thiamine when measured at the same pH.Pm-X was found to promote the growth of BKA, similarly to thiamine as well as other pyrimidine compounds having an amino group on the C4-position of pyrimidine. These findings have led to the conclusion that the isolated pyrimidine compound consisted certainly of the pyrimidine moiety of thiamine and possibly of a base, the nature of which remains to be settled.

• 日本ビタミン学会の論文

著者

• Murata Kiku

  Food And Nutrition Laboratories Teikoku Women's University

• MORITA JUNKO

  Food and Nutrition Laboratory, Faculty of Science of Living, Osaka City University

関連論文

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• THE DETERMINATION OF VITAMIN B12 ACTIVITY IN HUMAN BLOOD
• THE PYRIMIDINE PART OF THIAMINE DECOMPOSITION PRODUCT BY BACTERIAL THIAMINASE I
• Comparison of the Activity of the Tryptophan-NAD Pathway between Rats Fed a Fat-free Diet and a Fat Diet
• Effects of an Intraperitoneal Injection of a Large Amount of Nicotinamide on NAD and Thiamine in Livers of Thiamine-deficient Mice

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