好塩性細菌Vibrio alginolyticusの生長を促進するクロレラ抽出物中の活性物質〔英文〕
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The effect of hot water extract (CE, I) of chlorella cells on the growth and protein synthesis of Vibrio alginolyticus was studied and compared with its effect on of Vibrio parahaemolyticus, a slightly halophilic bacterium, and Escherichia coli, a non-halophilic bacterium. A remarkable growth-promoting effect of CE was found for V. alginolyticus but not for E. coli. The CE was fractionated by Sephadex G-25 column chromatography into two fractions (II and III). Fraction II was composed of high molecular weight (above 5, 000) compounds, and fraction III of low molecular weight (below 5, 000) compounds. Fraction II contained amino acids in higher concentration than fraction III, but it contained a smaller amount of S-containing amino acids. Fraction III exhibited a marked stimulatory effect on the growth of V. alginolyticus; fraction II did not show such a remarkable effect. This effect of fraction III might be due to easily utilizable amino acids, especially the S-containing amino acids contained in the fraction. Fraction II was further fractionated into four fractions (IV, V, VI, and VII) by DEAE-cellulose column chromatography. Fractions IV, V, and VI contained neutral sugar and protein in relatively high concentrations, but nucleic acids in very low concentration. Fraction VII showed the highest concentration of nucleic acids among the four fractions. Fractions V and VII had a growth-stimulating effect, but fractions IV and VI did not. Fraction VII showed the most remarkable effect on the growth and protein synthesis of V. alginolyticus. Based on these results, it is suggested that (1) high molecular weight fractions of CE, which contain thymine, adenine, guanine, cytosine and uracil as their main components, play an important role in the stimulation of growth and protein synthesis of V. alginolyticus, and that (2) amino acids in the low molecular weight fraction, especially S-containing amino acids, also stimulate the growth.
- 日本水産學會の論文
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- 好塩性細菌Vibrio alginolyticusの生長を促進するクロレラ抽出物中の活性物質〔英文〕