ヒジキにヒ素(5),ヒ素(3),メチルアルソン酸塩およびジメチルアルシン酸塩の確認

概要

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An aqueous extract from a brown seaweed, Hizikia fusiforme, was partially purified by benzene-extraction after being treated with alcohol for removal of polysaccharides. The extract was then subjected to strong-acid cation-exchange chromatography which consisted of a sercial connection of two columns of AG 50W×12. There arsenic fractions corresponding to the reference arsenate, arsenite and methylarsonate were obtained by elution of the column, with M/20 hydrochloric acid. Elution of the short column, taken apart from the long column, with 1.5M aqueous ammonia gave an additional arsenic fraction corresponding to the reference dimethylarsinate. These fractions were chromatographed on cellulose thin layer with a variety of solvent systems after being purified by gel filtration chromatography on Bio Gel P-2. By the t.l.c and co-t.l.c with the reference arsenicals, they were confirmed to be arsenate, arsenite, methylarsonate and dimethylarsinate, respectively.
日本水産學會の論文