夜盗蛾由来の細胞系におけるキヂヌ成長ホルモンの発現〔英文〕

概要

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A complementary DNA (564 base pairs) encoding for the mature region of the growth hormone (GH) of yellowfin porgy Acanthopagrus latus was amplified by polymerase chain reaction. This resultant DNA fragment, ypmGH cDNA, was inserted into baculovirus expression vectors, pAc7, under the control of the polyhedrin promoter. A 22 kDa protein, similar to that of authentic GH, was expressed intracellularly by the insect cell line of Spodoptera frugiperda 21-AE infected by recombinant baculovirus. This protein was reactive with rabbit polyclonal antibodies raised against natural chum salmon GH. Furthermore, a protein band with 22 kDa labeled with [35S] methionine was detected in tha lysate of cells infected with recombinant virus containing ypmGH eDNA. Results from treatment with tunicamycin, an inhibitor of N-link glycosylatíon, demonstrated that the N-glycosylation did not occur in the recombinant GH produced by this system.
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