鰓弁採取法で得た微量血漿試料の免疫化学試験によるサケ科魚種の早期雌雄判別法の開発と利用

概要

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Immunochemical methods to distinguish female and male salmonid fish prior to genital maturation with anti-vitellogenin (Vg) antibody have been developed. Diluted plasma of each fish was subjected to enzyme-linked immunosorbent assay (ELISA) using rabbit antiserum raised against Vg purified from the plasma of estradiol-injected male Oncorhynchus mykiss (rainbow trout) and the sex was tested with 100% accuracy in June, 4 months before spawning. Even in April or May, the method was applied with 80% accuracy. The present method required less than 2 ?? 3, μl of the blood sample, which we collected by picking up a piece of gill filament from individual fish and placing it in phosphate-buffered saline. By employing a dot-blotting method of ELISA on a polysulfone membrane, the test was completed in about 4 hours. The present method using anti-rainbow trout Vg can be used for other fish whose Vg cross-reacted with this antibody, for example, Oncorhynchus masou (yamame in Japanese) and Salvelinus leucomaenis pluvius (iwana in Japanese).
日本水産學會の論文