乾のりの核酸関連物質の分析〔イオン交換クロマトグラフィ-による核酸関連物質の分析-4-〕

概要

論文の詳細を見る
Nucleoside 5'-phosphates of AMP, CMP, GMP, IMP, UMP, nucleoside 2'-or 3'- phosphates of AMP, CMP, GMP, UMP, and ADP contained in dried purple laver Porphyra tenrea were separated and determined by anion exchange chromatography. The analysis was carried out using a column of φ9mm×220mm packed with Hitchi Model 2630 anion exchanger. Also, nucleosides, purine and pyrimidine bases in dried purple laver were eluted and collected at an early stage of anion exchange chromatography to remove nucleotides. And then adenine, guanine, adenosine, cytidine, and uridine in the fractionated solution were separated and determined by cation exchange chromatography. The analysis was carried out using a coumn of φ9mm×400mm packed with Hitachi Model 2613 cation exchanger. The amount of 5'-AMP was as much as compared with the other nucleoside 5'-phosphates, being 46-73mg/100g in dried purple laver. Both 5'-GMP and 5'-IMP were little, and the amount of the former was 6-13mg/100g and that of the latter was 9-11mg/100g. Most of the nucleotides in dried purple laver occurred in the form of nucleoside 2'-or 3'-phosphates, and nucleoside 5'-phosphates were few. Also, very small amouts of nucleosides, purine and pyrimidine bases were observed in dried purple laver.
日本水産學會の論文