培養細胞により免疫されたニジマスの細胞傷害性リンパ球に関する観察〔英文〕

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Prelimienary observaitions of the occurrence of cytotoxic cells was performed using the peripheral lymphocytes of juvenile rainbow trout Salmo gairdneri immunized BF-2 cells (a lined cell culture esablished from bluegill Lopomis macrochirus)intraperitoneally. Circulating lymtion, were inoculated into BF-2 and RTG-2 (a lined cell culture established from rainbow trout)cell cultures in tissus culture flasks. Cytotoxic effects of lymphocytes were qualitatively observed under phase-contrast microscopy after incubation at 15°C fot 1 day. While no cytotoxic effect of separated lymphocytes on RTG-2 cells was produced, the lymphocytes on day 5, 10 and 15 prior to immunzation revealed cytotoxic activity exclusively against BF-2 cells. In particular the lymphocytes on day 10 represented strong activity. The results suggested the occurrence of cytotoxic effector lymphocytes in response to hererologous antigenic cells in immunized rainbow trout.
日本水産學會の論文