コイ筋肉ホスホリラ-ゼbキナ-ゼの精製とその触媒的性質
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概要
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Procedures are described for purification of phosphorylase b kinase (EC. 2.7.1.38.) form carp muscle. The phosphorylase b kinases separated into two active fractions (Peak I and Peak II) on phospho-cellulose column chromatography by the linear gradient of KCl have similar properties with pH-depedency of activity, effect of Ca2+ on activity and Km values for ATP and phosphorylase b. Both kinases have a high ratio of activities at pH 6.8/pH 8.2 of 0.3-0.4 in comparison with a ratio of 0.05-0.08 for rabbit muscle enzyme.
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