酸化リノレン酸メチル多量体とジイソプロピルホスホリルトリプシンとの反応により生成した複合体〔英文〕

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The authors studied the reaction of diisopropylphosphoryltrypsin (DIP-trypsin), whose properties are quite similar to those of trypsin except for being enzymatically inactive, with the polymer of oxidized methyl linolenate (oxd MLn) as a model system of the reaction of trypsin with the polymer. By the reaction a considerable amount of the insoluble substance was produced from DIP-α- and DIP-β-trypsin. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis pattern showed that the insoluble substance consisted of DIP-trypsin monomer but not of DIP-trypsin polymerized by the intermolecular cross-linking among DIP-trypsin. Moreover, it was found that 2.6 to 3.9 mol of the polymer bound covalently per mol of DIP-trypsin by using [14C]polymer and by chromatography on Sepharose 4B in the presence of SDS.
社団法人日本水産学会の論文